• Citer

Translocation events demonstrated by molecular, in situ hybridization and chromosome pairing analyses in highly asymmetric somatic hybrid plants

par Hinnisdaels, Stefan;Mouras, Armand;Salesses, Georges;Veuskens, Jacky T M J.;Taylor, Christopher;Gharti-Chhetri, G.B.;Negrutiu, Ioan ;Jacobs, Michel
Référence Transgenic research, 1, 4, page (170-176)
Publication Publié, 1992-07

Article révisé par les pairs

• Détails
• Contenu

Résumé :

Cytological analyses show rearranged chromosomes in some highly asymmetric nuclear hybrids obtained after fusion of mesophyll protoplasts of Nicotiana plumbaginifolia (wild type) with γ-irradiated (100 krad), kanamycin-resistant mesophyll protoplasts of Petunia hybrida. Molecular, cytogenetic and in situ hybridization analyses performed on the asymmetric somatic hybrid P1, previously identified as having a clearly metacentric chromosome besides a nearly complete Nicotiana chromosome complement, are reported. Meiotic analysis and in situ hybridization experiments using ribosomal DNA as a probe showed that this metacentric chromosome represents a translocation of a chromosome fragment onto chromosome 9 of N. plumbaginifolia. Southern hybridization with an rDNA probe showed that only Nicotiana-specific rDNA was present. In situ hybridization experiments, using total genomic DNA of P. hybrida as a probe, demonstrated that the translocated fragment represented Petunia DNA. © 1992 Chapman & Hall.