• Citer

The effect of Beta-mercaptoethanol on the incorporation of precursors of proteins and nucleic acids into mouse liver and intestine.

par Brachet, Jean ;MIRAUX-JONCKHEERE, A
Référence Experimental cell research, 27, page (539-547)
Publication Publié, 1962-09

Article révisé par les pairs

• Détails
• Contenu

Résumé :

1. 1. We have studied the effects of an intraperitoneal injection of β-mercaptoethanol (2 mg/20 g body weight) on nucleic acid and protein synthesis in the liver and intestine of mice. The incorporation of 3H-thymidine, 3H-cytidine and 3H-leucine has been followed by autoradiography. 2. 2. The experiments have shown that β-mercaptoethanol inhibits mitotic activity and incorporation of thymidine into DNA in the intestine. 3. 3. The incorporation of cytidine and that of leucine are also strongly inhibited in the intestine as well as in the liver. The inhibition is of the same order of magnitude for the nucleus and the cytoplasm (except for cytidine, in the case of intestinal nuclei). In the liver, the incorporation of both precursors is higher in the nucleus than in the cytoplasm, while the opposite situation holds for the intestine. 4. 4. The significance of these results is briefly discussed. © 1962.