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Groupes sulfydriles et morphogénèse: III. Etude biochimique des effets du mercaptoéthanol sur les embryons de batraciens et l'algue Acetabularia mediterranea

par Brachet, Jean ;Decroly, Marc ;Hanocq Quertier, Jacqueline
Référence Developmental biology, 6, 1, page (113-131)
Publication Publié, 1963-02

Article révisé par les pairs

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Résumé :

The biochemical effects of β-mercaptoethanol (which inhibits neural tube closure very effectively) have been studied in amphibian gastrulae and neurulae, and in the alga Acetabularia. It has been found that: 1. 1. Total and acid soluble -SH groups definitely increase in mercaptoethanol-treated eggs. 2. 2. S35-Mercaptoethanol is incorporated into the proteins of both amphibian embryos and Acetabularia. The radioactivity of the proteins represents 25% of the total radioactivity in the treated eggs and varies between 10 and 25%, according to culture conditions, in Acetabularia. 3. 3. Autoradiography observations and work on homogenates have shown that S35-mercaptoethanol is incorporated mainly into the pigment granules and yolk platelets of the animal half and, later, of the dorsal half of the embryo. In Acetabularia, the incorporation is highest in the nucleus and in the apical part of the stem. 4. 4. It was not possible to detect by electrophoresis the appearance of a new soluble -SH-containing protein in mercaptoethanol-treated eggs. Most of the radioactivity is found in the basic protein fraction after treatment with S35-mercaptoethanol of amphibian eggs. 5. 5. Amphibian eggs contain no ecto-ATPase, and mercaptoethanol treatment has no appreciable effect on their endo-ATPase. The significance of these results for an explanation of the morphostatic effects of mercaptoethanol is discussed. © 1963.