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Deoxyribonucleic acid-dependent incorporation of amino acids into the proteins of chloroplasts isolated from anucleate acetabularia fragments.

par Goffeau, A;Brachet, Jean
Référence Biochimica et biophysica acta, 95, page (302-313)
Publication Publié, 1965-02

Article révisé par les pairs

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Résumé :

Isolated chloroplasts from anucleate fragments of Acetabularia actively incorporate a mixture of radioactive amino acids into proteins. This incorporation is inhibited by chloramphenicol and puromycin but appears to be relatively insensitive to streptomycin, ribonuclease (EC 2.7.7.16) and deoxyribonuclease (EC 3.1.4.5). Actinomycin D, at a concentration of 166 μg/ml, totally inhibits the incorporation of amino acids by chloroplasts of algae enucleated 11 days previously. It is concluded that, at this time, the synthesis of chloroplastic proteins is dependent on chloroplastic deoxyribonucleic acid. © 1965.