par De Marco, Federico;Hallez, Sophie ;Brulet, Jean-Marc ;Gesche, Fabienne ;Marzano, Pasqualina;Flamini, Silvio;Marcante, Maria Luisa;Venuti, Aldo
Référence Anticancer research, 23, 2 B, page (1449-1454)
Publication Publié, 2003-03
Référence Anticancer research, 23, 2 B, page (1449-1454)
Publication Publié, 2003-03
Article révisé par les pairs
Résumé : | Background: Genetic immunisation induces the endogenous production of the encoded antigens, which favours their presentation by MHC class I molecules. The E7 protein from "high risk" Human Papillomavirus (HPV) is constitutively expressed in cervical cancer and represents a target for immunotherapy. Materials and Methods: Several E7-encoding DNA vaccines were constructed including unmodified E7 and E7 fused to ubiquitin or to the Invariant chain in order to increase the presentation of E7-derived peptides by MHC class I or II molecules, respectively. These vaccines were administered i.m. to C57BL/6 mice that were subsequently challenged with E7-positive tumour cell lines expressing different levels of MHC class I molecules. Results: The E7-Ii fusion sequence protected a number of animals from tumour challenging. No differences were associated with the MHC class I status of the challenging cell lines. Conclusion: Engineering the intracellular pathway for antigen presentation is able to produce a valid therapeutic response even against tumours with down-regulated MHC class I. |