par Gregoire, Paul 
;Vermeulen, Claudine ;Ameryckx, Jean Pierre
Référence Biochimica et biophysica acta (G). General subjects, 279, 1, page (102-117)
Publication Publié, 1972-08
;Vermeulen, Claudine ;Ameryckx, Jean Pierre Référence Biochimica et biophysica acta (G). General subjects, 279, 1, page (102-117)
Publication Publié, 1972-08
Article révisé par les pairs
| Résumé : | Beef liver dermatan sulfate can be separated into two distinct fractions by electrophoresis on cellulose acetate. The faster moving Fraction II contains more sulfate. Both fractions are digested by chondroitinase ABC. Fraction I thereby liberates only Suzuki's ΔDi-4S disaccharide (J. Biol. Chem., 235 (1968) 3580) which is digestible with chondro-4-sulfatase. The same disaccharide is set free from Fraction II which yields moreover a saccharide which should be sulfated at position 6 of its hexosamine since it is digestible with chondro-6-sulfatase but differs from all known split products liberated by chondroitinase ABC. Dermatan sulfates isolated from beef lung and from rabbit skin contain only sulfated polysaccharide molecules similar to those of Fraction I. On digestion none of the dermatan sulfates examined gave rise to Suzuki's ΔDi-6S saccharide (J. Biol. Chem., 235 (1968) 3580). Beef liver heparitin sulfate differs in several respects from beef lung heparitin sulfate described by Dietrich et al. (Biochim. Biophys. Acta, 237 (1971) 430). Fractions obtained by column chromatography from beef liver heparitin sulfate show a continuously varying composition. While one at least of these fractions differs from the others by its electrophoretic mobility on cellulose acetate, beef liver heparitin sulfate does not yield distinct fractions when submitted to electrophoresis on an agarose gel. © 1972. |
