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Resistance to the aminoglycoside antibiotic neamine in Escherichia coli. A new mutant whose Nea(R) phenotype results from the cumulative effects of two distinct mutations

par Delcuve, Geneviève;Cabezón, Teresa ;Herzog, Albert
Référence Biochemical journal, 174, 1, page (1-7)
Publication Publié, 1978

Article révisé par les pairs

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Résumé :

A spontaneous mutant of E. coli (strain AB2847), selected for resistance to the aminoglycoside antibiotic neamine, shows severe restriction of amber suppressors in vivo. Ribosomes isolated from the mutant exhibit only low misreading in vitro in the presence of the antibiotic. Genetic and biochemical analyses indicate that the neamine-resistant phenotype is the result of two distinct mutations. The first, res3128, appears to affect the gene (strA) coding for the ribosomal protein S12. Although it leads to a restrictive phenotype it does not, however, confer resistance to streptomycin. The second mutation, X3128, is located between the strA and aroB loci and is lethal when segregated from the res3128 mutation. It may affect the ribosome at the level of a post-translational modification.