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Histological mapping of biochemical changes in solid tumors by FT-IR spectral imaging.

par Petibois, Cyril;Drogat, Benjamin ;Bikfalvi, Andreas;Déléris, Gérard;Moenner, Michel
Référence FEBS letters, 581, 28, page (5469-5474)
Publication Publié, 2007-11

Article révisé par les pairs

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Résumé :

Fourier-transform infrared (FT-IR) spectral imaging was used for analyzing biochemical changes in tumor cells. Metabolic parameters of human lung A549/8 adenocarcinoma and U87 glioma cells were compared under stress conditions in culture along with tumor progression after cell implantation onto the chick embryo chorio-allantoic membrane. In cell culture, glucose consumption and lactic acid release were higher in U87 cells. A549/8 cells were less sensitive to oxidative stress as observed through changes in fatty acyl chains. In vivo biochemical mapping of highly (U87) vs. poorly (A549/8) angiogenic tumors provided results comparable to culture models. Therefore, FT-IR imaging allows detecting subtle chemical changes in tumors, which might be useful for diagnosis.