• Citer

Comparative study of the determination of parabens in shampoos by liquid chromatography with amperometric and coulometric detection

par Kauffmann, Jean-Michel ;Murakami, Kazuo;Watanabe, H.;Tateno, T.
Référence Electroanalysis, 22, page (1720)
Publication Publié, 2010

Article révisé par les pairs

• Accès en ligne
• Détails
• Contenu
• Statistiques

Résumé :

The simultaneous determination of four para-hydroxybenzoic acid esters (parabens) in shampoos was studied by liquid chromatography (LC) with amperometric (LC-AD) and coulometric (LC-CD) detection. The parabens were separated on an ODS C18 reversed column by isocratic elution with a mobile phase based on methanol-0.1M acetic acid (60:40%, v/v) with 0.02M NaClO4 at a flow rate of 0.8 mL min-1. The limit of detection (S/N>3) for the analytes was in the 15 -25 pg (injected mass) range at an applied potential of 1.20 V vs. Ag/AgCl using the LC-AD and in the 2 -3 pg range at a potential of 0.790 V vs. Pd using the LC-CD. The peak ratio of the internal standard peak (IS: 4-hydroxybenzoic acid secbutyl ester) versus the analyte peak was found to be related to the amount injected from 0.1 ng to 100ng (r=0.996 -0.999) with the LC-AD and from 0.050 ng to 100 ng range (r=0.999 -1.000) with the LC-CD. The relative standard deviation (RSD, n=10) was comprised between 1.8 to 3.5% by LC-AD (5 ng injected) and between 2.0 to 2.4% by LC-CD (0.5 ng injected). The determination of four most used parabens in ten different shampoos was successfully realized. © 2010 Wiley-VCH Verlag GmbH&Co. KGaA, Weinheim.