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Analysis and modelling of Pseudomonas aeruginosa adherence to human buccal epithelial cells

par Cerf, Anne ;Dehaye, Jean-Paul ;Devleeschouwer, Michel
Référence Colloids and surfaces. B, Biointerfaces, 8, 4-5, page (267-277)
Publication Publié, 1997-04

Article révisé par les pairs

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Résumé :

In this paper we present a detailed analysis scheme of the bacterial adherence process which permits the modelling of this phenomenon. The binding of Pseudomonas aeruginosa ATCC 15442 onto human buccal epithelial cells (BECs) was studied. We performed adherence assays in the presence of increasing concentrations of P, aeruginosa, and we constructed a binding isotherm which was transformed into a Scatchard plot. The aspect of the Scatchard plot led us to consider two differential models describing the adherence of P. aeruginosa to BECs: the one class of binding sites model and the two classes of binding sites model. Our aim was to determine the model which best describes the experimental points. To this end, the binding isotherm was first regenerated on the basis of the known equations describing the two considered models and the values of the parameters of the equations estimated from the Scatchard plot. The two regenerated binding isotherms were then adjusted to the data points using a non-linear regression program. The model which presented the best fit was selected by performing an F test. We concluded that the two binding sites model fits the experimental data significantly better than the one binding site model.