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Capillary gel electrophoresis: Separation of major erythrocyte membrane proteins

par Lin, Changying ;Cotton, Frédéric ;Boutique, Charles ;Dhermy, Didier;Vertongen, Françoise ;Gulbis, Béatrice
Référence Journal of chromatography. B, Biomedical sciences and applications, 742, 2, page (411-419)
Publication Publié, 2000-06

Article révisé par les pairs

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Résumé :

A new separation method of human erythrocyte membrane proteins by sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE) is described. In this method, a replaceable gel matrix was used. Seven major erythrocyte membrane proteins, α-and β-spectrin, ankyrin 2.1, band 3 (anion-exchanger), 4.1a and b, and 4.2 (pallidin), were separated and identified by SDS-CGE method. High reproducible migration times of these proteins (inter-assay coefficients of variation less than 2%), as well as quantification (inter-assay coefficients of variation less than 11%) were obtained. This new SDS-CGE method may provide important diagnostic evidence for hereditary spherocytosis. It can be a powerful diagnostic tool in place of SDS polyacrylamide gel electrophoresis for erythrocyte membrane protein analysis. Copyright (C) 2000 Elsevier Science B.V.