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Comparison of culture and qPCR for the detection of Pseudomonas aeruginosa in not chronically infected cystic fibrosis patients.

par Deschaght, Pieter;Schelstraete, Petra;Lopes dos Santos Santiago, Guido;Van Simaey, Leen;Haerynck, Filomeen;Van Daele, Sabine;De Wachter, Elke;Malfroot, Anne;Lebecque, Patrick;Knoop, Christiane ;Casimir, Georges ;Boboli, Hedwige;Pierart, Frédéric;Desager, Kristine;Vaneechoutte, Mario;De Baets, Frans
Référence BMC Microbiology, 10, page (245)
Publication Publié, 2010

Article révisé par les pairs

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Résumé :

Pseudomonas aeruginosa is the major respiratory pathogen causing severe lung infections among CF patients, leading to high morbidity and mortality. Once infection is established, early antibiotic treatment is able to postpone the transition to chronic lung infection. In order to optimize the early detection, we compared the sensitivity of microbiological culture and quantitative PCR (qPCR) for the detection of P. aeruginosa in respiratory samples of not chronically infected CF patients.