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Chemical agents and enzymes used for the extraction of gut lymphocytes influence flow cytometric detection of T cell surface markers.

par Van Damme, Nicole ;Baeten, D;De Vos, Marc ;Demetter, Pieter ;Elewaut, D;Mielants, H;Verbruggen, G;Cuvelier, Claude A;Veys, E M;De Keyser, Frédérique
Référence Journal of immunological methods, 236, 1-2, page (27-35)
Publication Publié, 2000-03

Article révisé par les pairs

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Titre:	Chemical agents and enzymes used for the extraction of gut lymphocytes influence flow cytometric detection of T cell surface markers.
Auteur:	Van Damme, Nicole; Baeten, D; De Vos, Marc; Demetter, Pieter; Elewaut, D; Mielants, H; Verbruggen, G; Cuvelier, Claude A; Veys, E M; De Keyser, Frédérique
Informations sur la publication:	Journal of immunological methods, 236, 1-2, page (27-35)
Statut de publication:	Publié, 2000-03
Sujet CREF:	Anatomopathologie
	Médecine pathologie humaine
MeSH keywords:	Adolescent
	Adult
	Antigens, CD -- metabolism
	Cell Membrane -- drug effects -- immunology
	Cell Separation -- methods
	Child
	Child, Preschool
	Collagenases -- pharmacology
	Deoxyribonucleases -- pharmacology
	Dithiothreitol -- pharmacology
	Edetic Acid -- pharmacology
	Flow Cytometry -- methods
	Humans
	Indicators and Reagents
	Integrins -- metabolism
	L-Selectin -- metabolism
	Middle Aged
	Palatine Tonsil -- cytology -- immunology
	T-Lymphocytes -- cytology -- drug effects -- immunology
Note générale:	In Vitro
	Journal Article
	Research Support, Non-U.S. Gov't
Langue:	Anglais
Identificateurs:	urn:issn:0022-1759
	info:pii/S0022-1759(99)00243-4
	info:pmid/10699577