Résumé : This study compares the performance of three Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) systems: Microflex LT (Bruker Daltonics, Bremen, Germany), Vitek MS RUO (Axima Assurance - Saramis database; bioMérieux, Marcy l'Etoile, France), and Vitek MS IVD (bioMérieux).A total of 1,129 isolates including 1,003 routine isolates, 73 anaerobes, and 53 bacterial enteropathogens were tested on the Microflex LT and Axima Assurance devices. The spectra were analyzed using three databases: Biotyper (Bruker Daltonics), Saramis, and Vitek MS (bioMérieux).Among the routine isolates requiring identification to the species level (n=986), 92.7% and 93.2% were correctly identified by the Biotyper and Vitek MS databases, respectively. The Vitek MS database is more specific for the identification of S. viridans. For the anaerobes, the Biotyper database often only identified Fusobacterium isolates to the genus level, which is of low clinical significance, whereas 20% of the Bacteroides species were not identified or were misidentified by the Vitek MS database. For the enteropathogens, the poor discrimination between E. coli and Shigella explains the high proportion of unidentified organisms. In contrast to the Biotyper database, the Vitek MS database properly discriminated all of the S. typhi isolates (n=5). The performance of the Saramis database was globally poorer.In conclusion, for routine procedures, the Microflex LT and Vitek-MS systems are equally good choices in terms of their analytical efficiency. Other factors, including price, workflow and lab activity, will affect the choice of a system.