par Vekemans, Marcel ;Robyn, Claude
Référence European Journal of Obstetrics and Gynecology and Reproductive Biology, 4, 3, page (111-123)
Publication Publié, 1974
Article révisé par les pairs
Résumé : The dynamics of the immunologic reactions involved in a double antibody radioimmunoassay for HLH were investigated in order to set up a rapid assay. Classically, assays were conducted in 5 or 6 days at 4°C. Shortening the incubation of unlabelled HLH with anti HCG serum decreases the sensitivity of the assay, whereas reduction of the second incubation with labelled HCG enhances it. However, a too short second incubation results in insufficient binding of the labelled hormone. With first and second incubations of 2 and 1 hr, respectively, 10 mIU of HLH decrease the amount of labelled hormone bound to the antibody by 35%. Immunoprecipitation of the hormone antihormone complexes by the second antibody was faster in buffer containing 20% human serum than in buffer alone: respectively, 80% and 10% of precipitation are obtained within 1 hr. A rapid radioimmunoassay was standardized, reducing the 3 incubations to 2, 1 and 1 hr, respectively. All incubations were conducted at 37°C. Sensitivity varied around 1.5 mIU. Index of precision was 0.19. Within and between assay variations were 13.0 and 14.6%. Results obtained with classical and rapid assays were highly correlated.