par Smeesters, Pierre 
;Drèze, Pierre-Alexandre ;Bousbata, Sabrina ;Parikka, Kaarle J;Timmery, Sophie;Hu, Xiaomin;Perez-Morga, David ;Deghorain, Marie ;Toussaint, Ariane ;Mahillon, Jacques;Van Melderen, Laurence
Référence Research in microbiology, 162, 4, page (446-459)
Publication Publié, 2011-05
;Drèze, Pierre-Alexandre ;Bousbata, Sabrina ;Parikka, Kaarle J;Timmery, Sophie;Hu, Xiaomin;Perez-Morga, David ;Deghorain, Marie ;Toussaint, Ariane ;Mahillon, Jacques;Van Melderen, Laurence Référence Research in microbiology, 162, 4, page (446-459)
Publication Publié, 2011-05
Article révisé par les pairs
| Résumé : | A novel temperate bacteriophage was isolated from a Bacillus cereus cereulide-producing strain and named vB_BceS-IEBH. vB_BceS-IEBH belongs to the Siphoviridae family. The complete genome sequence (53 kb) was determined and annotated. Eighty-seven ORFs were detected and for 28, a putative function was assigned using the ACLAME database. vB_BceS-IEBH replicates as a plasmid in the prophage state. Accordingly, a 9-kb plasmid-like region composed of 13 ORFs was identified. A fragment of around 2000 bp comprising an ORF encoding a putative plasmid replication protein was shown to be self-replicating in Bacillus thuringiensis. Mass spectrometry analysis of the purified vB_BceS-IEBH particle identified 8 structural proteins and enabled assignment of a supplementary ORF as being part of the morphogenesis module. Genome analysis further illustrates the diversity of mobile genetic elements and their plasticity within the B. cereus group. |
