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Acarbose treatment or islet transplantation increase FAD-glycerophosphate dehydrogenase content in islets of diabetic rats

par Fabregat, Marta E;Fernandez-Álvarez, Josefa;Franco, Carmen;Corominola, Helena;Mas, E;Malaisse, Willy ;Gomis, Ramon
Référence Medical science research, 27, page (393-396)
Publication Publié, 1999

Article révisé par les pairs

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Résumé :

Male adult rats injected with streptozotocin during the neonatal period (STZ rats) showed low body weight, high fluid intake, increased glycaemia, low plasma insulin concentration, decreased islet content in FAD-glycerophosphate dehydrogenase (mGDH), low activity of mGDH in islet homogenates, low islet insulin content and decreased secretory responsiveness of the islets to D-glucose, as compared to control animals of same sex and age. When the STZ rats were given access to acarbose (40 mg/100 g of food) for one month, the fluid intake, glycaemia, pancreatic islet content in mGDH and activity of this enzyme in islet homogenates were all improved, but the plasma insulin concentration remained unchanged. Likewise, when the STZ rats were grafted with 1000-1200 islets from normal rats, the mGDH content of pancreatic islets again increased. This now coincided with a significant increase in plasma insulin concentration, but a somewhat lower increment (Δ) in insulin output, in response to a rise in D-glucose concentration from 2.8 to 16.7 mmol/l, in the islets from transplanted rats (Δ = 21.4 ± 6.2 μU/islet per 90 min) than in those from untreated STZ rats (Δ = 39.5 ± 8.8 μU/islet per 90 min). By comparison with data obtained in other experimental models of B-cell dysfunction, these findings suggest that sustained hyperglycaemia unfavourably affects expression of the mGDH gene in pancreatic islets of animals suffering from a primary alteration of their insulin-secreting cell population.