Résumé : Forests play a critical role in mitigating climate change by capturing and storing carbon dioxidefrom the atmosphere. The objective of this study was to estimate the carbon stock of woodyplants in the Gullele Botanical Garden along environmental gradients. Data were collected on30 quadrant plots, each 20 x 20 meters in size, distributed along transect lines. Breast heightdiameter ≥ 5 cm) and height of each tree in the main plot were measured. Carbon content indifferent plants components such as above-ground biomass, below-ground biomass and litterbiomass were measured. Additionally, soil organic carbon was also analyzed. The resultsshowed significant variations in carbon stocks along environmental gradients. Above-ground,below-ground, and litter carbon stocks showed a decreasing trend with an increasing elevationand slope. On the other hand, soil organic carbon stocks increased with increasing elevation.The mean above-ground carbon stock was estimated to be 176 t C ha-1, below-ground carbonstock was estimated to be 35.26 t C ha-1, litter carbon stock was estimated to be 1.79 t C ha-1,and soil organic carbon stock was 347.15 t C ha-1. Above-ground, below-ground, and littercarbon stocks are higher in southern areas and lower in northern areas, while soil organiccarbon stocks are higher in the north than northwest. The study concludes that GulleleBotanical Garden Store large amount of carbon in different carbon pools of the forest andvaries according to the environmental gradient.