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Intra- and interspecific variation in genome size of the coral genus Agaricia from Curaçao

par Rañises, Dina Mae
Promoteur Flot, Jean-François
Co-Promoteur Malay, Maria Celia;VERMEIJ, Mark
Publication Non publié, 2022-08-31

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Résumé :

Genome sizeisafundamentalbiologicaltraitthatisknowntoexhibithighdiversityamongeukaryoticspecies,butitsintraspecificdiversityhasonlyscarcelybeenstudiedtodate.Inscleractiniancorals,genomesizedataareonlyavailable forafewspecies.Inthisstudy,intra-andinterspecificvariationingenome sizeofthecoralgenus Agaricia collected fromCuraçaowereinvestigated. MorphologywascongruentwithgeneticanalysesofthenuclearmarkerITS2indelimitingthreeAgariciaspeciesamongoursamples.ArefinedFeulgenImageAnalysisDensitometry(FIAD)protocolyieldedgenomesizesthatranged from0.359pgto0.593pgwithinthisgenus(a1.6-foldrange).Thehighest averagegenomesizewasrecordedin A. agaricites (0.496 pg,1.2-foldrange), followedby A. lamarcki (0.460 pg,1.5-foldrange)and A. humilis (0.429pg, 1.3-foldrange).Theaveragegenomesizeof A. agaricites wassignificantlylargerthantheothertwospecies.Nocorrelationbetweenaveragegenomesizeand nucleotidepolymorphism π wasdetected,butwefoundanalmostlinearcorrelationbetweenintraspecificvarianceofgenomesizeand π (p =0.079).Genome sizeandcollectiondepthsofboth A. lamarcki and A. agaricites wasalso notsignificantlyassociated.Toourknowledge,thisstudyprovidesthefirstaccount ofintraspecificvariationincoralsandtheapparentcorrelationdetectedbetweenthenucleotidepolymorphismofaspeciesandthevarianceofitsgenome sizewillhavetobetestedusingalargertaxonomicspectrumofscleractinian coralsaswellasinothergroupsofanimals.