par Miljević, Iva 
Président du jury Dahdouh-Guebas, Farid
Promoteur PAPINI, Alessio
Co-Promoteur Thilahgavani, Nagappan;Adibah Abdul Mahid, Fadzidlah
Publication Non publié, 2021-08-31
Président du jury Dahdouh-Guebas, Farid
Promoteur PAPINI, Alessio
Co-Promoteur Thilahgavani, Nagappan;Adibah Abdul Mahid, Fadzidlah
Publication Non publié, 2021-08-31
Mémoire
| Résumé : | Terpenoids represent some of the most diverse and valuable classes of chemical compounds. They play a major role in plants' defense and pollinating system, thus ensuring plant's viability. Being one of the ancient phyla that helps us to understand the evolution of plants, cycads have always gained special attention in research. Some of the rarest and commercially most valuable species belong to Encephalartos, a genus native to the south of Africa. Throughout the research, the focus was on localizing terpenoids within the anatomical structures of the leaf as well as a chemical assay of two Encephalartos species with very different ecological features, E. arenarius and E. munchii. To check for differences between these species, we used fresh leaf material from the Botanical garden of Florence. Results of anatomical localization using NADI reagent showed no difference in terpenoid location between plants resulting in positive detection in the upper (adaxial) epidermis, the upper layer of mesophyll cells, as lipid bodies in spongy parenchyma and some crystal structures. Chemical analysis using GC-MS showed quantitative and qualitative differences between the two species, with E. munchii having a prevalent number of overall compounds as well as detected terpenoids. The obtained results are consistent with the ecology of the target species. |
