par Nikko, Elina 
;Marini, Anna Maria ;André, Bruno
Référence The Journal of biological chemistry, 278, 50, page (50732-50743)
Publication Publié, 2003-12
;Marini, Anna Maria ;André, Bruno Référence The Journal of biological chemistry, 278, 50, page (50732-50743)
Publication Publié, 2003-12
Article révisé par les pairs
| Résumé : | Ubiquitination of the yeast Gap1 permease at the plasma membrane triggers its endocytosis followed by targeting to the vacuolar lumen for degradation. We previously identified Bro1 as a protein essential to this down-regulation. In this study, we show that Bro1 is essential neither to ubiquitination nor to the early steps of Gap1 endocytosis. Bro1 rather intervenes at a late step of the multivesicular body (MVB) pathway, after the core components of the endosome-associated ESCRT-III protein complex and before or in conjunction with Doa4, the ubiquitin hydrolase mediating protein deubiquitination prior to their incorporation into MVB vesicles. Bro1 markedly differs from other class E vacuolar protein sorting factors involved in MVB sorting as lack of Bro1 leads to recycling of the internalized permease back to the plasma membrane by passing through the Golgi. This recycling seems to be accompanied by deubiquitination of the permease and unexpectedly requires a normal endosome-to-vacuole transport function. |
