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A protein domain-based interactome network for C. elegans early embryogenesis.

par Boxem, Mike;Maliga, Zoltan;Klitgord, Niels;Li, Nan;Lemmens, Irma;Mana, Miyeko;de Lichtervelde, Lorenzo;Mul, Joram D;van de Peut, Diederik;Devos, Maxime;Simonis, Nicolas ;Yildirim, Muhammed A;Cokol, Murat;Kao, Huey-Ling;de Smet, Anne-Sophie;Wang, Haidong;Schlaitz, Anne-Lore;Hao, Tong;Milstein, Stuart;Fan, Changyu;Tipsword, Mike;Drew, Kevin;Galli, Matilde;Rhrissorrakrai, Kahn;Drechsel, David;Koller, Daphne;Roth, Frederick P;Iakoucheva, Lilia M;Dunker, A Keith;Bonneau, Richard;Gunsalus, Kristin C;Hill, David E;Piano, Fabio;Tavernier, Jan;van den Heuvel, Sander;Hyman, Anthony A;Vidal, Marc
Référence Cell, 134, 3, page (534-545)
Publication Publié, 2008-08

Article révisé par les pairs

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Résumé :

Many protein-protein interactions are mediated through independently folding modular domains. Proteome-wide efforts to model protein-protein interaction or "interactome" networks have largely ignored this modular organization of proteins. We developed an experimental strategy to efficiently identify interaction domains and generated a domain-based interactome network for proteins involved in C. elegans early-embryonic cell divisions. Minimal interacting regions were identified for over 200 proteins, providing important information on their domain organization. Furthermore, our approach increased the sensitivity of the two-hybrid system, resulting in a more complete interactome network. This interactome modeling strategy revealed insights into C. elegans centrosome function and is applicable to other biological processes in this and other organisms.