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Identification of 1-lysophosphatidylethanolamine (C16:1) as an antimicrobial compound in the housefly, Musca domestica

par Meylaers, Karen;Clynen, Elke;Daloze, Désiré ;DeLoof, Arnold;Schoofs, Liliane
Référence Insect biochemistry and molecular biology, 34, 1, page (43-49)
Publication Publié, 2004-01

Article révisé par les pairs

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Résumé :

We observed that a methanolic whole body extract of uninfected last instar larvae of the housefly, Musca domestica, displayed antifungal and antibacterial activity. We have further purified this extract to a single active fraction using reversed phase high performance liquid chromatography. The pure fraction inhibited growth of the Gram-positive bacteria Bacillus thuringiensis and the yeast Saccharomyces cerevisiae, but not the Gram-negative bacteria Escherichia coli. The active compound was determined to have a molecular mass of 451.2 Da. Further analysis by nuclear magnetic resonance identified the substance as mono-unsaturated 1-lysophosphatidylethanolamine (C16:1) (1-LPE). The structurally different and more common 2-LPE have been described as mediators of the antimicrobial activity of rimenophenazine antibiotic agents (Van Rensburg et al., 1992). Our results suggest that the isolated 1-LPE displays a higher activity in comparison, possibly based on structure-specific differences in activity. © 2003 Elsevier Ltd. All rights reserved.