• Citer

The use of sea urchin egg as a model to investigate the effects of Crassolide, a diterpene isolated from a soft coral

par Pesando, D.;Graillet, C.;Braekman, Jean Claude ;Dubreuil, A.;Girard, J. P.;Puiseux-Dao, Simone
Référence Toxicology in vitro, 5, 5-6, page (395-401)
Publication Publié, 1991

Article révisé par les pairs

• Accès en ligne
• Détails
• Contenu
• Statistiques

Résumé :

Crassolide, a monocyclic diterpene isolated and purified from the soft coral Lobophytum crassum, inhibited the cell cleavage of sea urchin eggs without affecting fertilization. The effect was observed with concentrations above 2 × 10-5 m in egg suspensions. Addition of crassolide between 5 and 40 min post-fertilization totally blocked the first cleavage, which in the control occurs 1 hr after fertilization. When added between 50 and 60 min post-fertilization, crassolide produced polynucleated cells in embryos. Crassolide did not affect the egg permeability to Na+ and Ca2+, but caused an increase of 0.2 units in the intracellular pH of fertilized eggs coupled with a proton efflux. Crassolide, which does not affect Ca2+ influx or permeability at the level of storage in reticular vesicles, could be used as a negative control when analysing calcium changes in short-term toxicological studies. The relationship between the pH increase and the cell cleavage needs further investigation. © 1991.