par Opdecamp, Karin 
;Riviere, Michèle ;Molné, Montse ;Szpirer, Josiane ;Szpirer, Claude
Référence Nucleic acids research, 20, 2, page (171-178)
Publication Publié, 1992-01
;Riviere, Michèle ;Molné, Montse ;Szpirer, Josiane ;Szpirer, Claude Référence Nucleic acids research, 20, 2, page (171-178)
Publication Publié, 1992-01
Article révisé par les pairs
| Résumé : | By comparing the methylation pattern of Mspl/Hpall sites in the 5' region of the mouse alpha-foetoprotein (AFP) gene of different cells (hepatoma cells, foetal and adult liver, fibroblasts), we found a correlation between gene expression and unmethylation of a site located in the first intron of the gene. Other sites did not show this correlation. In transfection experiments of unmethylated and methylated AFP-CAT chimeric constructions, we then showed that methylation of the intronic site negatively modulates expression of CAT activity. We also found that a DNA segment centered on this site binds nuclear proteins; however methylation did not affect protein binding. |
