par Arcone, R;Fontaine, Véronique ;Coto, I;Brakenhoff, Just P.;Content, Jean ;Ciliberto, G
Référence FEBS letters, 288, 1-2, page (197-200)
Publication Publié, 1991-08
Référence FEBS letters, 288, 1-2, page (197-200)
Publication Publié, 1991-08
Article révisé par les pairs
Résumé : | Internal deletions of the human interleukin-6 (IL-6) cDNA have been generated in the region encoding residues 29 to 42. Mutant proteins were produced by in vitro transcription-translation or in Escherichia coli and tested for their biological activity using the hybridoma growth factor (HGF) assay or a transcriptional activation assay on human hepatoma cells. The folding of the mutants was also checked by immunoprecipitation with conformation-specific monoclonal antibodies. The results show that only residues 29 to 34 are crucial for IL-6 activity and that the first two amino acids are probably involved in the definition of the IL-6 active site. |