One-step purification of mouse monoclonal antibodies from ascitic fluid by DEAE Affi-gel blue chromatography

Bruck, Claudine; Portetelle, Daniel; Glineur, Cécile; Bollen, Alex

doi:doi/10.1016/0022-1759(82)90178-8

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One-step purification of mouse monoclonal antibodies from ascitic fluid by DEAE Affi-gel blue chromatography

par Bruck, Claudine

;Portetelle, Daniel

;Glineur, Cécile

;Bollen, Alex

Référence Journal of immunological methods, 53, 3, page (313-319)
Publication Publié, 1982

Article révisé par les pairs

Résumé :

Monoclonal antibodies can be purified directly from ascitic fluids by chromatography on a DEAE Affi-gel blue column. Optimal conditions were determined for the recovery of immunoglobulins free of contaminating protease and nuclease activities.

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