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Correct in vivo processing of a chimeric ubiquitin-proapolipoprotein A-I fusion protein in baculovirus-infected insect cells

par Moguilevsky, Nicole ;Guillaume, Jean Paul ;Varsolana, F.;Bulinckx, Michel;Bollen, Alex
Référence Journal of biotechnology, 32, 1, page (39-43)
Publication Publié, 1994

Article révisé par les pairs

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Résumé :

The cDNA coding for human proapolipoprotein A-I was expressed as a ubiquitin fusion under the control of the polyhedrin promoter in baculovirus-infected Sf9 Spodoptera frugiperda insect cells. The fusion protein was expressed at high level and was quantitatively cleaved in vivo. The cleaved product ws purified and its N-terminal amino acid sequence was established. The data showed that authentic proapolipoprotein A-I has been produced, and thus demonstrated the existence in Spodoptera frugiperda insect cells of a specific ubiquitin hydrolase activity.