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Effect of introduction of an Arginine16 in VIP, PACAP and secretin on ligand affinity for the receptors

par Gourlet, Philippe ;Vandermeers, André ;Vandermeers-Piret, Marie-Claire ;De Neef, Philippe ;Waelbroeck, Magali ;Robberecht, Patrick
Référence Biochimica et biophysica acta. Molecular cell research, 1314, 3, page (267-273)
Publication Publié, 1996-12

Article révisé par les pairs

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Résumé :

Rabbit secretin, which differs from all other mammalian secretins in having a Leu residue in position 6 (instead of Phe) and a basic residue (Arg) in position 16, had a lower affinity than porcine secretin on recombinant rat secretin receptors but had a greater affinity than porcine secretin on recombinant rat VIP1 aud PACAP I receptors. Synthetic [L6] porcine secretin had a reduced potency on secretin and VIP1 receptors whereas [R16] porcine secretin had a similar binding profile as rabbit secretin. Thus, an arginine residue in position 16 reduced 3-fold the affinity of secretin for secretin receptors but increased 30-fold its affinity for the VIP1 and PACAP I receptors. The introduction of an arginine residue in position 16, instead of glutamine, in VIP and PACAP had a similar effect: [R16] VIP and [R16] PACAP had 3- to 10-fold higher affinities than VIP and PACAP for VIP1 and PACAP I receptors, and 3-fold lower affinities for the secretin receptors. The three [R16] peptides also had a reduced potency on the chimeric receptor consisting of the N-terminal part of the secretin receptor grafted on the VIP1 receptor, and an enhanced potency on the chimeric receptor consisting of the N-terminal part of VIP1 receptor grafted on the secretin receptor, indicating that position 16 of each ligand interacted with the N-terminal extracellular domain of the receptors.