Résumé : The study of the determination of the overnight interest rate in the interbank market, and the behaviour of its volatility, gained new insights with contributions from the microstructure theory. The aim of this article is to study the effect of the trade intensity over the volatility of the overnight interest rate, using intra-daily data related to the Portuguese Money Market (MMI). The analysis is focused in two different periods of time, before and after the introduction of the minimum reserve rules of the Single Monetary Policy. We find that these rules have contributed to interest rate stability.