par Ruysschaert, Jean Marie 
;El Ouahabi, Abdel;Willeaume, Véronique ;Huez, Georges ;Fuks, Robert ;Vandenbranden, Michel ;Di Stefano, Patrick
Référence Biochemical and biophysical research communications, 203, 3, page (1622-1628)
Publication Publié, 1994-09
;El Ouahabi, Abdel;Willeaume, Véronique ;Huez, Georges ;Fuks, Robert ;Vandenbranden, Michel ;Di Stefano, Patrick Référence Biochemical and biophysical research communications, 203, 3, page (1622-1628)
Publication Publié, 1994-09
Article révisé par les pairs
| Résumé : | We describe here a new cationic amphiphile, N-t-butyl-N'-tetradecyl-3-tetradecylaminopropionamidine (diC14-amidine), which interacts with plasmid DNA and generates hydrophobic stable complexes resistant against DNase I. In partition experiments between two non-miscible phases, DNA was transferred into an organic phase upon complex formation with diC14-amidine-containing vesicles. Finally, vesicles made of a diC14-amidine and phosphatidylethanolamine (PE) (1:1, mol:mol) mixture or pure diC14-amidine were efficient in mediating transfection of adherent (CHO) and suspension (K562) cell lines, using the chloramphenicol acetyltransferase (CAT) gene as reporter. |
