par Scruel, Olivier ;Sener, Abdullah ;Malaisse, Willy
Référence Journal of chromatography, 847, 1-2, page (53-57)
Publication Publié, 1999-06
Article révisé par les pairs
Résumé : This paper describes the microsynthesis and the purification of various 14C- or 3H-labelled tracers of D-fructose. These tracers were prepared from the corresponding 14C- or 3H-tracers of D-glucose by interconversion in alkaline media, and then purified by HPLC using a carbohydrate column constituted of a calcium-loaded sulfonated polymer. This work also documents that the purification step is essential to investigate in a reliable manner the metabolism of D-fructose in isolated rat pancreatic islets. Copyright (C) 1999 Elsevier Science B.V.

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