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Effects of a medium-chain triglyceride:fish oil emulsion administered intravenously to omega3 fatty acid-depleted rats on cationic fluxes in aortic rings.

par Courtois, Philippe ;Louchami, Karim ;Portois, Laurence ;Chardigny, Jean-Michel;Sener, Abdullah ;Carpentier, Yvon ;Malaisse, Willy
Référence International Journal of Molecular Medicine, 16, 6, page (1089-1093)
Publication Publié, 2005

Article révisé par les pairs

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Résumé :

The handling of 45Ca and 86Rb by aortic rings obtained from rats depleted in long-chain polyunsaturated omega3 fatty acids (second generation) was examined in vitro over 10 to 60 min incubation at either increasing concentrations of extracellular K+ (5, 3 and 60 mM) in the case of 45Ca net uptake or in the absence and presence of ouabain (50 microM) in the case of 86Rb uptake. The omega3-depleted rats were injected intravenously 120 min before sacrifice with 1.0 ml of either an omega3 fatty acid-rich medium-chain triglyceride:fish oil emulsion (MCT:FO) or a control medium-chain triglyceride:olive oil emulsion (MCT:OO). In the MCT:OO-injected rats, the rise in extracellular K+ concentration failed to stimulate 45Ca net uptake, whilst the prior injection of the MCT:FO emulsion restored the expected increase in 45Ca net uptake by aortic rings exposed to 60 mM K+. The absolute value for 86Rb net uptake after 10 or 60 min incubation and whether in the absence or presence of ouabain, which significantly decreased the uptake of 86Rb+ after 60 min incubation, only represented in the MCT:FO-injected rats 63.1+/-3.8% of the mean corresponding values found in MCT:OO-injected animals. These findings are consistent with the view that activity of cationic channels, such as the voltage-sensitive Ca2+ channel, the outflow of Ca2+ as mediated by either Na+-Ca2+ countertransport or a Ca2+-ATPase, the activity of Na+,K+-ATPase and the modality of K+ inflow by an oubain-resistant modality are all affected in aortic cells by the content of long-chain polyunsaturated omega3 fatty acids in membrane phospholipids.