par Pector, Véronique 
;Backmann, J.;Maes, Didier ;Vandenbranden, Michel ;Ruysschaert, Jean Marie
Référence The Journal of biological chemistry, 275, 38, page (29533-29538)
Publication Publié, 2000-09
;Backmann, J.;Maes, Didier ;Vandenbranden, Michel ;Ruysschaert, Jean Marie Référence The Journal of biological chemistry, 275, 38, page (29533-29538)
Publication Publié, 2000-09
Article révisé par les pairs
| Résumé : | Cationic liposomes are used as vectors for gene delivery both in vitro and in vivo. Comprehension of both DNA/liposome interactions on a molecular level and a description of structural modifications involved, are prerequisites to an optimization of the transfection protocol and, thus, successful application in therapy. Formation and stability of a DNA/cationic liposome complex were investigated here at different DNA:lipid molar ratios (rho). Isothermal titration calorimetry (ITC) of cationic liposomes with plasmid DNA was used to characterize the DNA-lipid interaction. Two processes were shown to be involved in the complex formation. A fast exothermic process was attributed to the electrostatic binding of DNA to the liposome surface. A subsequent slower endothermic reaction is likely to be caused by the fusion of the two components and their rearrangement into a new structure. Fluorescence and differential scanning calorimetry confirmed this interpretation. A kinetic model analyzes the ITC profile in terms of DNA/cationic liposome interactions. |
