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Distinct regulation by lipopolysaccharides of the expression of interleukin-1{beta} by murine macrophages and salivary glands.

par Seil, Michèle ;El Ouaaliti, Malika ;Abdou Foumekoye, S;Pochet, Stéphanie ;Dehaye, Jean-Paul
Référence Innate Immunity, 18, page (14-24)
Publication Publié, 2012

Article révisé par les pairs

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Résumé :

The regulation of interleukin (IL)-1 expression and secretion by salivary glands and macrophages in response to lipopolysaccharides (LPS) was compared. In wild-type mice, injection of LPS significantly decreased the volume of saliva stimulated by pilocarpine and increased its protein and amylase concentration. It did not modify the salivary concentration of IL-1beta. The cytokine was expressed by submandibular acini and ducts. Macrophages also expressed IL-1beta but at lower concentration than salivary glands. The pre-incubation of macrophages with LPS increased the phosphorylation of IkappaB and the expression of IL-1beta. Adenosine triphosphate also promoted the secretion of the cytokine by these cells. These responses were absent in submandibular gland cells. These glands expressed CD14, TLR4 and MyD88. P2X7-KO mice secreted a lower volume of saliva which contained less proteins and amylase. In conclusion, IL-1beta is constitutively expressed by submandibular glands and its secretion is not regulated by a P2X7 agonist. In these cells, LPS do not activate the nuclear factor-kappaB-pro-IL-1beta axis in spite of the expression of the proteins involved in their recognition.