par Droogmans, Louis 
;Cludts, Isabelle ;Cleuter, Yvette ;Kettmann, Richard ;Burny, Arsène
Référence DNA Sequence - Journal of DNA Sequencing and Mapping, 3, 2, page (115-117)
Publication Publié, 1992
;Cludts, Isabelle ;Cleuter, Yvette ;Kettmann, Richard ;Burny, Arsène Référence DNA Sequence - Journal of DNA Sequencing and Mapping, 3, 2, page (115-117)
Publication Publié, 1992
Article révisé par les pairs
| Résumé : | We report the cloning and sequencing of a 1252 base pairs (bp) DNA fragment containing the bovine interleukin-6 (IL-6) gene promoter. This fragment was isolated from a bovine genomic library constructed in the lambda GEM11 vector. Comparison with human, murine and rat IL-6 gene promoters reveals a high degree of conservation of the 200 bp immediately upstream of the RNA CAP site. This region contains nucleotide stretches matching with consensus sequences recognized by transcription factors, including NF-KB, CREB and NF-IL6. A potential AP-1 binding site is found 284 nucleotides upstream of the RNA CAP site. The bovine IL-6 Gene promoter cloned upstream of the bacterial chloramphenicol acetyl transferase (CAT) Gene was shown to be active in bovine and ovine cells. © 1992 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted. |
