par Horuk, R;Hesselgesser, J;Zhou, Y;Faulds, D;Halks-Miller, M;Harvey, S;Taub, D;Samson, M;Parmentier, Marc 
;Rucker, J;Doranz, Benjamin J.;Doms, Robert W.
Référence The Journal of biological chemistry, 273, 1, page (386-391)
Publication Publié, 1998-01
;Rucker, J;Doranz, Benjamin J.;Doms, Robert W.Référence The Journal of biological chemistry, 273, 1, page (386-391)
Publication Publié, 1998-01
Article révisé par les pairs
| Résumé : | Using a chemokine receptor model based on known receptor sequences, we identified several members of the seven transmembrane domain G-protein superfamily as potential chemokine receptors. The orphan receptor ChemR1, which has recently been shown to be a receptor for the CC chemokine I-309, scored very high in our model. We have confirmed that I-309, but not a number of other chemokines, can induce a transient Ca2+ flux in cells expressing CCR8. In addition, the human erythroleukemic cell line K562 responded chemotactically in a dose-responsive manner to this chemokine. Since several chemokine receptors have been shown to be required as coreceptors for HIV-1 infection, we asked whether human immunodeficiency virus type 1 (HIV-1) could efficiently utilize CCR8. Here we show that the CCR8 receptor can serve as a coreceptor for diverse T-cell tropic, dual-tropic, and macrophage-tropic HIV-1 strains and that I-309 was a potent inhibitor of HIV-1 envelope-mediated cell-cell fusion and virus infection. Furthermore, we show by flow cytometry and immunohistochemistry that antibodies generated against the CCR8 receptor amino-terminal peptide cross-reacted with U-87 MG cells stably expressing CCR8, THP-1 cells, HL-60 cells, and human monocytes, a target cell for HIV-1 infectivity in vivo. |
