par Pochet, Roland 
;Delespesse, Guy ;Gausset, Philippe ;Collet, Henry
Référence Clinical and experimental immunology, 38, 3, page (578-584)
Publication Publié, 1979-12
;Delespesse, Guy ;Gausset, Philippe ;Collet, HenryRéférence Clinical and experimental immunology, 38, 3, page (578-584)
Publication Publié, 1979-12
Article révisé par les pairs
| Résumé : | A technique is described allowing the quantification and the characterization of specific beta-adrenergic receptors in intact living human lymphocytes. 125I-Iodohydroxybenzylpindolol, a potent beta-adrenergic antagonist was used to label specific binding sites on unfractionated lymphoid cells and on purified subpopulations of T (F1 and F2) and B cells. F1 and F2 were obtained by filtration through nylon wool column as previously described (Delespesse et al., 1976), they differ in their response to mitogens, and in their interactions with adherent cells and B cells. 125I-HYP binding to unfractionated lymphocytes was a saturable, stereospecific and rapid process with a dissociation constant of 2.5 10(-10) M and a binding capacity of 400--600 sites/cell. Bindings on unfractionated lymphocytes, purified B cells and T cells of the F2 fraction were similar. No detectable binding was noted on T cells from the F1 fraction. Enriched T cells obtained by a rosetting technique displayed 200 receptors/cell. |
