par Laurent, M;Pays, Etienne 
;Delinte, K;Magnus, Eddy;Van Meirvenne, N;Steinert, Maurice
Référence Nature (London), 308, 5957, page (370-373)
Publication Publié, 1984
;Delinte, K;Magnus, Eddy;Van Meirvenne, N;Steinert, Maurice Référence Nature (London), 308, 5957, page (370-373)
Publication Publié, 1984
Article révisé par les pairs
| Résumé : | African trypanosomes activate, one at a time, a large set of genes coding for different variant-specific surface antigens (VSAs). These genes have been classed into two groups. In the first group a permanently silent basic gene copy is duplicated and the expression-linked copy (ELC) transposed to an expression site located at a chromosome end. The process is a gene conversion which changes a variable stretch of the preceding ELC. Genes belonging to the second group do not give rise to an additional copy when expressed by a still unknown mechanism. We report here that the gene for antigenic type AnTat 1.6 is located in a telomeric DNA region and is expressed without being duplicated. In clone AnTat 1.6 and the ensuing ones, the ELC of the preceding VSA (AnTat 1.3) is conserved, but in a inactive conformation. Moreover, the AnTat 1.6 gene is lost from the genome of the AnTat 1.6-derived variants, in which the duplication-linked mechanism of gene activation occurs: the gene appears to be replaced by the incoming ELC. These observations show that a trypanosome surface antigen repertoire may evolve by loss and gain of VSA genes, depending on the alternation of the different recombinational mechanism involved in antigenic variation. |
