Purification and characterization of two voltage-dependent anion channel isoforms from plant seeds.

Abrecht, H; Wattiez, R; Ruysschaert, Jean Marie; Homblé, Fabrice

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Purification and characterization of two voltage-dependent anion channel isoforms from plant seeds.

par Abrecht, H ;Wattiez, R ;Ruysschaert, Jean Marie

;Homblé, Fabrice

Référence Plant physiology, 124, 3, page (1181-1190)
Publication Publié, 2000-11

Article révisé par les pairs

Résumé :

Mitochondria were isolated from imbibed seeds of lentil (Lens culinaris) and Phaseolus vulgaris. We copurified two voltage-dependent anion channel from detergent solubilized mitochondria in a single purification step using hydroxyapatite. The two isoforms from P. vulgaris were separated by chromatofocusing chromatography in 4 M urea without any loss of channel activity. Channel activity of each isoform was characterized upon reconstitution into diphytanoyl phosphatidylcholine planar lipid bilayers. Both isoforms form large conductance channels that are slightly anion selective and display cation selective substates.

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