Identification of a minor Tg mRNA transcript in RNA from normal and goitrous thyroids.

Targovnik, H. M.; Cochaux, Pascale; Corach, Daniel; Vassart, Gilbert

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Identification of a minor Tg mRNA transcript in RNA from normal and goitrous thyroids.

par Targovnik, H. M.;Cochaux, Pascale

;Corach, Daniel ;Vassart, Gilbert

Référence Molecular and cellular endocrinology, 84, 1-2, page (R23-R26)
Publication Publié, 1992-03

Article révisé par les pairs

Résumé :

Polymerase chain reaction (PCR) amplification of nt 4502 to nt 5184 of the thyroglobulin (Tg) mRNA from several patients, with or without elevated serum thyrotropin (TSH), showed a predominant fragment of the expected size (683 bp) and a minor fragment of 512 bp. The sequence of this minor fragment revealed that 171 bp were missing between position 4567 and 4737. It is highly probable that the deleted sequence corresponds to a complete exon, suggesting an alternative splicing as mechanism for the generation of the minor transcript.

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