par Bulur, Nurdan
;Virreira Bermudez, Myrna
;Soyfoo, Muhammad Shahnawaz
;Louchami, Karim
;Delporte, Christine
;Perret, Jason
;Beauwens, Renaud
;Malaisse, Willy
;Sener, Abdullah 
Référence Cellular physiology and biochemistry, 24, 3-4, page (187-192)
Publication Publié, 2009









Référence Cellular physiology and biochemistry, 24, 3-4, page (187-192)
Publication Publié, 2009
Article révisé par les pairs
Résumé : | BACKGROUND/AIMS: The expression of the electrogenic Na+-HCO3--cotransporter NBCe1 was recently documented in rat pancreatic islet B-cells, it being speculated that such a protein participates in the extrusion of bicarbonate generated by the oxidative catabolism of nutrients from insulin-producing cells. Considering the prevalence of a Crabtree effect in tumoral insulin-producing cells, the possible presence of NBCe1 was now investigated in BRIN-BD11 cells, an insulin-producing cell line established by electrofusion of normal pancreatic B-cells with immortalized RINm5F cells. METHODS: The possible presence of NBCe1 in BRIN-BD11 cells was investigated by RT-PCR, western blot analysis and immunocytochemistry. The release of insulin and net uptake of 22Na+ were also measured in the BRIN-BD11 cells. RESULTS: RT-PCR, western blot analysis and immunocytochemistry documented the presence of NBCe1 in BRIN-BD11 cells. A reported inhibitor of NBCe1, i.e. tenidap, (50-100 microM), inhibited basal and hypotonicity-induced insulin release from the BRIN-BD11 cells, whilst increasing the net uptake of 22Na+ by the same cells. The latter effect was, in relative terms, more pronounced in the presence than absence of ouabain. CONCLUSION: BRIN-BD11 cells, like normal pancreatic islet B-cells, express NBCe1, with predominance of the B variant of this electrogenic Na+-HCO3--cotransporter. |