• Citer

The human thyroglobulin gene contains two 15-17 kb introns near its 3'-end.

par van Ommen, G J;Arnberg, A C;Baas, F;Brocas, Huguette;Sterk, A;Tegelaers, W H;Vassart, Gilbert ;De Vijlder, J J M
Référence Nucleic acids research, 11, 8, page (2273-2285)
Publication Publié, 1983-04

Article révisé par les pairs

• Accès en ligne
• Détails
• Contenu
• Statistiques

Résumé :

We have cloned overlapping segments of the human thyroglobulin gene from a genomic cosmid library. Restriction mapping and electron microscopy show that a region of 38 kb at or near the 3'-end of this gene encodes only 850 nucleotides or 10% of the messenger RNA (mRNA) sequence. The region contains five exons of 130-210 nucleotides, split by introns of 1 to 15-17 kb. This represents the lowest ratio of coding to non-coding DNA (2.2%) found thus far in any eukaryotic gene. Blot hybridization under non-stringent conditions shows the presence of only one copy of this gene in the human genome and the absence of other closely related sequences.