Radioautographic localization of prolactin messenger RNA on histological sections by in situ hybridization.

Pochet, Roland; Brocas, Huguette; Vassart, Gilbert; Toubeau, Gérard; Seo, Hye-Sook; Refetoff, Samuel; Dumont, Jacques; Pasteels, Jean Lambert

doi:doi/10.1016/0006-8993(81)90969-0

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Radioautographic localization of prolactin messenger RNA on histological sections by in situ hybridization.

par Pochet, Roland

;Brocas, Huguette ;Vassart, Gilbert

;Pasteels, Jean Lambert

Référence Brain research, 211, 2, page (433-438)
Publication Publié, 1981-05

Article révisé par les pairs

Résumé :

In situ hybridization of complementary [H3]DNA ([H3]cDNA) synthetized from purified rat prolactin messenger RNA (rPRL mRNA) was performed to specifically identify on histologic sections of rat hypophysis cells expressing the PRL gene. Radioautographic labelling occurred over weakly acidophilic cells, while other acidophils, with darker cytoplasm did not contain more silver grains than blood vessels.

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