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Cloning and expression of human brain type I inositol 1,4,5-trisphosphate 5-phosphatase: high levels of mRNA in cerebellar Purkinje cells

par De Smedt, Florence ;Verjans, Benoît ;Mailleux, Pierre ;Erneux, Christophe
Référence FEBS letters, 347, 1, page (69-72)
Publication Publié, 1994-06

Article révisé par les pairs

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Résumé :

In brain and many other tissues, Type I inositol 1,4,5-trisphosphate (InsP3) 5-phosphatase is the major isozyme hydrolysing the calcium-mobilizing second messenger InsP3. We recently reported the cloning and expression of dog thyroid InsP3 5-phosphatase. During the course of this cloning, screening of a human brain cDNA library allowed us to isolate a cDNA clone D1 with 91% sequence identity with the thyroid sequence. When clone D1 was expressed in Escherichia coli, the fusion protein had InsP3 5-phosphatase activity. Mr estimates of the recombinant enzyme made by unmunodetection, activity assay after SDS/PAGE or silver staining were consistent with the calculated molecular mass. In situ hybridization on human cerebellum sections localised the mRNA for this enzyme to the Purkinje cells. © 1994.