par Belayew, Alexandra ;Bellefroid, Eric ;Berwaer, M;Dumoulin, Michel;Lambert, Christine;Lemaitre-Wathy, C;Mathy-Hartert, M;Morin, A;Pasleau, Françoise ;Scippo, M L
Référence Annales d'Endocrinologie, 47, 1, page (7-10)
Publication Publié, 1986
Référence Annales d'Endocrinologie, 47, 1, page (7-10)
Publication Publié, 1986
Article révisé par les pairs
Résumé : | We are studying nucleotide sequences responsible for the regulation of eukaryotic gene expression. Our test system comprises the human genes coding for prolactin (hPRL), growth hormone (hGH-N) and placental lactogen (hCS-B). We have cloned these genes and are searching within their sequences for in vitro binding sites of the human glucocorticoid receptor on the hGH-N and hCS-B genes; the in vivo activity of such DNA sequences by assaying hybrid gene expression in transfected cells; in vivo "enhancer" activity of different hPRL gene fragments linked to a marker gene and transfected in cultured cells. |