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Reliability of the tissue microarray based FISH for evaluation of the HER-2 oncogene in breast carcinoma.

par Gancberg, David ;Di Leo, Angelo ;Rouas, Ghizlane;Järvinen, Tero;Verhest, Alain;Isola, Jorma;Piccart-Gebhart, Martine ;Larsimont, Denis
Référence Journal of Clinical Pathology, 55, 4, page (315-317)
Publication Publié, 2002-04

Article révisé par les pairs

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Résumé :

AIMS: Tumour tissue microarray allows the analysis of hundreds of tumour samples simultaneously on a single microscope slide. However, the extremely small tissue samples taken from the original tissue may not always be representative of the entire tumour. METHODS: The reliability of this new technology was investigated by analysing HER-2 oncogene amplification by fluorescence in situ hybridisation (FISH) from representative slides of the whole tumour and small tissue core biopsies from 29 invasive breast tumours. RESULTS: The tissue microarray method had high accuracy; in only one of 29 cases (3.4%; 95% confidence interval, 0% to 10%) were the results discordant with whole tumour analysis. CONCLUSION: Tumour microarray is a highly reliable method for analysing HER-2 oncogene amplification by FISH in human breast tumours.