par George, Isabelle 
;Petit, M.;Servais, Pierre
Référence Journal of applied microbiology, 88, 3, page (404-413)
Publication Publié, 2000
;Petit, M.;Servais, Pierre Référence Journal of applied microbiology, 88, 3, page (404-413)
Publication Publié, 2000
Article révisé par les pairs
| Résumé : | Rapid enumeration methods based on the enzymatic hydrolysis of 4-methylumbelliferyl-β- d-galactoside and 4-methylumbelliferyl-β- d-glucuronide were optimized for freshwaters. The enzymes β- d-galactosidase (GALase) and β- d-glucuronidase (GLUase) were shown to be already induced in freshwaters when tested, respectively, with the inducers isopropyl-β- d-thiogalactopyranoside and methyl-β- d-glucuronide. Both enzymatic activities were compared, respectively, with plate counts of total and faecal coliforms in freshwaters. Enzymatic methods and reference plate counts were significantly correlated in log–log plots. Moreover, the GLUase method allowed the detection of viable (presenting a detectable GLUase activity) but nonculturable Escherichia coli. |
