Titre:
  • Expression and purification in high yield of a functionally active recombinant human Type I inositol(1,4,5)P3 5-phosphatase.
Auteur:Wohlkonig, Alexandre; Sénéchal, Magalie; Dewitte, Frédérique; Backers, Katrien; Erneux, Christophe; Villeret, Vincent
Informations sur la publication:Protein expression and purification, 55, 1, page (69-74)
Statut de publication:Publié, 2007-09
Sujet CREF:Sciences bio-médicales et agricoles
Mots-clés:Double affinity tags
Human recombinant protein
Purification
Type I human inositol 1,4,5-triphosphate 5-phosphatase
MeSH keywords:Carrier Proteins -- chemistry
Carrier Proteins -- genetics
Cloning, Molecular
Escherichia coli -- genetics
Genetic Vectors -- genetics
Humans
Phosphoric Monoester Hydrolases -- biosynthesis
Phosphoric Monoester Hydrolases -- genetics
Phosphoric Monoester Hydrolases -- isolation & purification
Recombinant Fusion Proteins -- biosynthesis
Recombinant Fusion Proteins -- chemistry
Recombinant Fusion Proteins -- isolation & purification
Note générale:Journal Article
Research Support, Non-U.S. Gov't
Langue:Anglais
Identificateurs:urn:issn:1046-5928
info:doi/10.1016/j.pep.2007.04.009
info:pii/S1046-5928(07)00108-8
info:scp/34547929313
info:pmid/17537645