par Gatot, Jean-Stéphane ;Gioia, Romain;Chau, Tieu-Lan;Patrascu, Félicia;Warnier, Michael;Close, Pierre;Chapelle, Jean-Paul;Muraille, Eric ;Brown, Keith;Siebenlist, Ulrich;Piette, Jacques;Dejardin, Emmanuel;Chariot, Alain
Référence The Journal of biological chemistry, 282, 43, page (31131-31146)
Publication Publié, 2007-10
Référence The Journal of biological chemistry, 282, 43, page (31131-31146)
Publication Publié, 2007-10
Article révisé par les pairs
Résumé : | Type I interferon gene induction relies on IKK-related kinase TBK1 and IKKepsilon-mediated phosphorylations of IRF3/7 through the Toll-like receptor-dependent signaling pathways. The scaffold proteins that assemble these kinase complexes are poorly characterized. We show here that TANK/ITRAF is required for the TBK1- and IKKepsilon-mediated IRF3/7 phosphorylations through some Toll-like receptor-dependent pathways and is part of a TRAF3-containing complex. Moreover, TANK is dispensable for the early phase of double-stranded RNA-mediated IRF3 phosphorylation. Interestingly, TANK is heavily phosphorylated by TBK1-IKKepsilon upon lipopolysaccharide stimulation and is also subject to lipopolysaccharide- and TBK1-IKKepsilon-mediated Lys(63)-linked polyubiquitination, a mechanism that does not require TBK1-IKKepsilon kinase activity. Thus, we have identified TANK as a scaffold protein that assembles some but not all IRF3/7-phosphorylating TBK1-IKKepsilon complexes and demonstrated that these kinases possess two functions, namely the phosphorylation of both IRF3/7 and TANK as well as the recruitment of an E3 ligase for Lys(63)-linked polyubiquitination of their scaffold protein, TANK. |